LOCUS       UGT3A1-pGEc2            6675 bp    DNA     circular     21-SEP-2016
FEATURES             Location/Qualifiers
     misc_feature    complement(6153..6282)
                     /note="3'-ITR"
     misc_feature    complement(5851..6065)
                     /note="BGH pA"
     rep_origin      1137..1804
                     /dnas_title="Col E1 origin"
                     /label=Col E1 origin
                     /vntifkey="33"
     CDS             129..989
                     /dnas_title="AmpR"
                     /label=AmpR
                     /vntifkey="4"
     misc_feature    1998..2127
                     /note="5'ITR"
     misc_feature    2208..2790
                     /note="CMV Promoter"
     misc_feature    2828..2960
                     /note="Artificial Intron"
     misc_feature    5303..5844
                     /note="WPRE"
     misc_recomb     complement(2974..3001)
                     /label=attB1
                     /note="attB1 site"
                     /note="recognized as attB1"
     misc_feature    4482..4504
                     /note="attB2"
     misc_feature    5282..5287
                     /note="BstB1 site"
     misc_feature    4505..5200
                     /note="Super GFP"
     misc_feature    5201..5251
                     /note="AviTag"
     misc_feature    5252..5275
                     /note="His Tag"
     CDS             3002..4481
                     /label=UGT3A1_Cterm
     misc_feature    3011..4459
                     /note="OPTIMIZED UGT3A1 coding region"
     misc_feature    4460..4481
                     /note="TEV site"
     vector          join(4490..6675,1..2991)
                     /source="pGEc2-DEST"
                     /type="Destination Vector"
     clone           2992..4489
                     /source="UGT3A1-pDONR"
                     /type="Entry Clone"
     source          1..6675
                     /dnas_title="UGT3A1-pGEc2"
ORIGIN      
        1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT
       61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA
      121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT
      181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT
      241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT
      301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG
      361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA
      421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA
      481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA
      541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA
      601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA
      661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA
      721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC
      781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC
      841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG
      901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA
      961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT
     1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA
     1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG
     1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA
     1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT
     1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC
     1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA
     1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA
     1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC
     1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA
     1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA
     1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG
     1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC
     1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG
     1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA
     1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA
     1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG
     1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG
     2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG
     2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT
     2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG
     2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT
     2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG
     2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT
     2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT
     2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG
     2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG
     2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC
     2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA
     2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC
     2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC
     2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT
     2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA
     2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC
     3001 TCGCGCCACC ATGGTCGGAC AGAGAGTGCT GCTGCTGGTC GCTTTTCTGC TGAGCGGAGT
     3061 CCTGCTGTCT GAGGCCGCCA AGATCCTGAC AATCTCTACA CTGGGCGGCA GCCACTACCT
     3121 GCTGCTCGAT AGAGTGTCCC AGATTCTGCA GGAACACGGC CACAACGTCA CCATGCTGCA
     3181 CCAGAGCGGC AAGTTTCTGA TCCCCGACAT CAAAGAGGAA GAGAAGTCCT ACCAGGTCAT
     3241 CCGGTGGTTC AGCCCCGAGG ACCACCAGAA GAGAATCAAG AAGCACTTCG ACAGCTACAT
     3301 CGAGACAGCC CTGGACGGCC GGAAAGAATC TGAAGCCCTG GTCAAGCTGA TGGAAATCTT
     3361 CGGCACCCAG TGCAGCTATC TGCTGTCCCG GAAGGACATC ATGGACAGCC TGAAGAACGA
     3421 GAACTACGAC CTGGTGTTCG TGGAAGCCTT CGATTTCTGC AGCTTCCTGA TCGCCGAGAA
     3481 GCTCGTGAAG CCATTCGTGG CCATCCTGCC TACCACCTTC GGCAGCCTGG ATTTTGGACT
     3541 GCCTAGCCCT CTGAGCTACG TGCCCGTGTT TCCTAGCCTG CTGACCGACC ACATGGATTT
     3601 CTGGGGCAGA GTGAAGAATT TCCTGATGTT CTTTAGCTTC AGCCGCAGCC AGTGGGACAT
     3661 GCAGAGCACC TTCGATAACA CTATCAAAGA ACACTTCCCC GAGGGCAGCA GACCCGTGCT
     3721 GTCTCATCTC CTGCTGAAGG CCGAGCTGTG GTTCGTGAAC AGCGACTTCG CCTTCGACTT
     3781 TGCCAGGCCC CTGCTGCCTA ATACCGTGTA TATCGGCGGA CTCATGGAAA AGCCCATCAA
     3841 GCCTGTGCCC CAGGACCTGG ACAACTTTAT CGCCAATTTC GGCGACGCCG GCTTCGTGCT
     3901 GGTGGCCTTT GGATCTATGC TGAACACCCA CCAGTCCCAG GAAGTGCTGA AGAAGATGCA
     3961 CAACGCCTTC GCCCATCTGC CTCAGGGCGT GATCTGGACC TGTCAGAGTA GCCACTGGCC
     4021 CAGAGATGTG CACCTGGCCA CCAACGTGAA GATCGTGGAT TGGCTGCCAC AGAGCGATCT
     4081 GCTGGCCCAC CCTAGCATCA GACTGTTTGT GACACACGGC GGCCAGAACA GCGTGATGGA
     4141 AGCTATTAGA CACGGGGTGC CAATGGTCGG CCTGCCTGTG AATGGGGACC AGCATGGAAA
     4201 TATGGTCCGA GTGGTGGCTA AGAATTACGG CGTGTCCATC CGGCTGAATC AGGTCACAGC
     4261 CGATACCCTG ACACTGACCA TGAAGCAGGT CATTGAGGAC AAGCGGTACA AGTCTGCCGT
     4321 GGTGGCCGCT TCTGTGATCC TGCATTCTCA GCCTCTGTCT CCAGCACAGA GACTCGTGGG
     4381 CTGGATCGAT CACATTCTCC AGACTGGCGG AGCCACCCAC CTGAAGCCTT ATGCTTTTCA
     4441 GCAGCCTTGG CACGAGCAGG AGAACCTGTA TTTTCAAGGC TCCAGCTTTC TTGTACAAAG
     4501 TGGTATGAGC AAGGGCGAGG AACTGTTCAC CGGCGTGGTG CCCATCCTGG TGGAACTGGA
     4561 TGGCGACGTG AACGGCCACA AGTTCTCTGT GCGGGGAGAG GGCGAAGGCG ACGCCACAAA
     4621 TGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CTTGGCCTAC
     4681 CCTCGTGACC ACACTGACCT ACGGCGTGCA GTGCTTCAGC AGATACCCCG ACCATATGAA
     4741 GCGGCACGAC TTCTTCAAGA GCGCCATGCC CGAGGGCTAC GTGCAGGAAC GGACCATCAG
     4801 CTTCAAGGAC GACGGCACCT ACAAGACCAG AGCCGAAGTG AAGTTCGAGG GCGACACCCT
     4861 CGTGAACCGG ATCGAGCTGA AGGGCATCGA TTTCAAAGAG GACGGCAACA TCCTGGGCCA
     4921 CAAGCTGGAG TACAACTTCA ACAGCCACAA CGTGTACATC ACCGCCGACA AGCAGAAGAA
     4981 CGGCATCAAG GCCAACTTCA AGATCCGGCA CAACGTGGAA GATGGCAGCG TGCAGCTGGC
     5041 CGACCACTAC CAGCAGAACA CCCCCATCGG AGATGGCCCC GTGCTGCTGC CCGACAACCA
     5101 CTACCTGAGC ACCCAGAGCG TGCTGAGCAA GGACCCCAAC GAGAAGCGGG ACCACATGGT
     5161 GCTGCTGGAA TTCGTGACCG CCGCTGGCAT CACCCACGGC ATGAGCGGCC TGAACGACAT
     5221 CTTCGAGGCC CAGAAAATCG AGTGGCACGA GCACCACCAT CATCACCATC ACCACTGATG
     5281 ATTCGAAAAA TAATAAGGAT CCAATCAACC TCTGGATTAC AAAATTTGTG AAAGATTGAC
     5341 TGGTATTCTT AACTATGTTG CTCCTTTTAC GCTATGTGGA TACGCTGCTT TAATGCCTTT
     5401 GTATCATGCT ATTGCTTCCC GTATGGCTTT CATTTTCTCC TCCTTGTATA AATCCTGGTT
     5461 GCTGTCTCTT TATGAGGAGT TGTGGCCCGT TGTCAGGCAA CGTGGCGTGG TGTGCACTGT
     5521 GTTTGCTGAC GCAACCCCCA CTGGTTGGGG CATTGCCACC ACCTGTCAGC TCCTTTCCGG
     5581 GACTTTCGCT TTCCCCCTCC CTATTGCCAC GGCGGAACTC ATCGCCGCCT GCCTTGCCCG
     5641 CTGCTGGACA GGGGCTCGGC TGTTGGGCAC TGACAATTCC GTGGTGTTGT CGGGGAAGCT
     5701 GACGTCCTTT CCATGGCTGC TCGCCTGTGT TGCCACCTGG ATTCTGCGCG GGACGTCCTT
     5761 CTGCTACGTC CCTTCGGCCC TCAATCCAGC GGACCTTCCT TCCCGCGGCC TGCTGCCGGC
     5821 TCTGCGGCCT CTTCCGCGTC TTCGAGATCT GCCTCGACTG TGCCTTCTAG TTGCCAGCCA
     5881 TCTGTTGTTT GCCCCTCCCC CGTGCCTTCC TTGACCCTGG AAGGTGCCAC TCCCACTGTC
     5941 CTTTCCTAAT AAAATGAGGA AATTGCATCG CATTGTCTGA GTAGGTGTCA TTCTATTCTG
     6001 GGGGGTGGGG TGGGGCAGGA CAGCAAGGGG GAGGATTGGG AAGACAATAG CAGGCATGCT
     6061 GGGGACTCGA GTTAAGGGCG AATTGCCGAT TAGGATCTTC CTAGAGCATG GCTACGTAGA
     6121 TAAGTAGCAT GGCGGGTTAA TCATTAACTA CAAGGAACCC CTAGTGATGG AGTTGGCCAC
     6181 TCCCTCTCTG CGCGCTCGCT CGCTCACTGA GGCCGGGCGA CCAAAGGTCG CCCGACGCCC
     6241 GGGCTTTGCC CGGGCGGCCT CAGTGAGCGA GCGAGCGCGC AGCCTTAATT AAGAGCTCAT
     6301 GGCGCGCCTA GGCCTTGACG GCCTTCCGCC AATTCGCCCT ATAGTGAGTC GTATTACGTC
     6361 GCGCTCACTG GCCGTCGTTT TACAACGTCG TGACTGGGAA AACCCTGGCG TTACCCAACT
     6421 TAATCGCCTT GCAGCACATC CCCCTTTCGC CAGCTGGCGT AATAGCGAAG AGGCCCGCAC
     6481 CGAAACGCCC TTCCCAACAG TTGCGCAGCC TGAATGGCGA ATGGGAGCGC CCTGTAGCGG
     6541 CCACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC
     6601 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTTAA CAAAATATTA
     6661 ACGCTTACAA TTTAG
//

